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One such acid-inducible gene encodes isocitrate lyase that converts isocitrate to succinate and glyoxylate ( + to make isonicotinoyl-NAD that inhibits mycolic acid synthesis, a bacterial cell wall component, subsequently interfering with mycobacterial cell wall integrity ( rpoB that encodes RNA polymerase B protein ( Figure 1 )

Besides protein purification, the HIC methodology offers several potentialities in protein production, being described as one of the most used strategies for endotoxin clearance (Wilson et al., 2001