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Regulation of hepatic glutathione metabolism and its role in hepatotoxicity

To this end, both WT-GPX4- and GPX4-2QA-expressing cells were treated with CHX in combination with the proteasome inhibitor MG132, the lysosomal inhibitor NHCl, or the autophagy inhibitor chloroquine (CQ) and harvested for western blot analysis 4 h later (Fig