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Several studies have reported that supplementing rats with both L-carnitine and alpha-lipoic acid halts age-related increases in reactive oxygen species (ROS), lipid peroxidation, protein carbonylation, and DNA strand breaks in heart, skeletal muscle and brain, concomitant with improvement in mitochondrial enzyme and respiratory chain activities [146149]

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[114] PacBio SMRT sequencing offers methylation detection as part of PCR-free DNA sequencing Oxford Nanopore Technologies DNA Sequencing can also be done with simultaneous Methylation calling if the DNA was not amplified by PCR prior to sequencing Differentially methylated regions (DMRs) [edit] Differentially methylated regions, which are genomic regions with different methylation statuses among multiple samples (tissues, cells, individuals or others), are regarded as possible functional regions involved in gene transcriptional regulation