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The membranes were blocked at 37C for 1.5 h in 5% milk and then incubated 12-16 h at 4C with the following primary antibodies: COX2 (Abcam, Cat# ab179800), AKR1C1 (Abcam, Cat# ab192785), FTH1 (Abcam, Cat# ab75973), GPX4 (Abcam, Cat# ab125066) and -actin (Invitrogen, Cat# A1978)

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Therefore, vials containing bacteriostatic water for injection should always be stored in a cool and dry place to ensure the integrity of the solution