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Reverse Transcription-Quantitative Polymerase Chain Reaction The total RNA content was isolated from cells or the tumor using TRIzol reagent (Invitrogen), treated with DNase I (Thermo Fisher Scientific, Cat# EN0521), and then reverse transcribed into cDNA using a Maximal First Strand cDNA Synthesis Kit (Thermo Fisher Scientific, Cat# K1622)

generated nanothin encapsulation materials (TA-PVP) for islet protection, which is composed of tannic acid (61), a polyphenolic compound with ROS scavenging and anti-inflammatory activities, and poly(N-vinylpyrrolidone)

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From 24 h pi until the end of the trial, a constant but significantly lower level than the initial one was maintained