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Furthermore, GHK-Cu effectively reduced immune cell infiltration and alveolar permeability, critical hallmarks of ALI

doi: 10.1007/s11270-021-05287-4 101 ChenC.-C.WuJ.LinT.-C

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Cells were incubated in TUNEL reaction mixture (Roche, Germany) at 37 C for one h, washed three times with PBS and incubated in blocking solution for 30 min at room temperature