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10.3389/fpls.2012.00292 172 ShigeokaS.IshikawaT.TamoiM.MiyagawaY.TakedaT.YabutaY.et al

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In particular, these include: (i) the differing expression environments of proteins in vitro (at different stages of the culture or with addition of blood for example) and in vivo (inside the tick, or in the host, at the beginning or the late infection), and (ii) the composition of the binding buffer such as the presence of detergents or salts