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To extract ABCC2 form cell membrane, cells were rotated gently for 2 h in 25 mM Tris-HCl pH 7.4, 150 mM NaCl, 20% (v/v) glycerol, 1% (w/v) n-Dodecyl--D-Maltopyranoside (DDM, Bluepus) and 0.2% (w/v) cholesteryl hemisuccinate (CHS, Anatrace)

doi: 10.2337/db12-0533 610 MorozNCarmonaJJAndersonEHartACSinclairDABlackwellTK

Pharmacotherapy includes calcium channel blockers or targeted prostacyclin, nitrate oxide, and endothelin pathways

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