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The only difference is how your protein of interest is connected to the agarose beads, which has important implications for how you elute it, and any proteins that it binds to from the column

Addissouky TA, El Tantawy El Sayed I, Wang Y

HLA-matched sibling donor HSCT may increase 2-year overall and event-free survival and reduce risk of extended chronic GVHD compared to other types of donor HSCT in patients with -TM (181)