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En ambos casos, las clulas del organismo no producen suficiente energa, lo que afecta principalmente a los rganos con mayores necesidades energticas, como el corazn, los msculos y el hgado

The next day, cells were transfected with 0.1 g plasmid per well using Lipofectamine 2000 (Thermo Fisher Scientific)

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Conversely, the effects of interacting cells, tissues and organ systems in vivo are unlikely to be recapitulated in 2D or 3D tissue models of skeletal muscle, even though flutamide + androgen treatment in those experiments would be targeted to muscle only