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Cell-free mercury(II)-reducing activity in a plasmid-bearing strain of Escherichia coli

Biochim Biophys Acta 2012;1823:143443

For droplet formation assays, the purified protein solution was mixed at a 1:1 ratio with PEG3350 buffer (100 M Bis-Tris pH 6.0 containing the indicated concentrations of PEG3350)

Except for cytochrome oxidase which retains the reduced form of oxygen intermediates to their active site, all the other elements of the mitochondrial respiratory chain transfer the electrons directly to oxygen and do not retain any reduced intermediate of oxygen at their active site to protect the cell against oxidative damages