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When stored sealed at room temperature, the product maintains stability for extended periods under recommended storage conditions

Slides were blocked in blocking solution [2% (w/v) BSA, 0.1% (v/v) Tween 20 in PBS] for 20 min and incubated with primary antibodies: rat monoclonal anti-BrdU (Abcam, ab6326

We acknowledge the utilization of this flexibility and recognize that many practitioners have stressed the importance of maintaining it, however we seek additional information regarding potential patient safety and quality of care concerns

Simultaneously, hypoxia impairs DC differentiation and antigen presentation by downregulating MHC class II and costimulatory molecules (CD80/CD86), 177 as well as suppressing IL-12 secretion, thereby weakening T-cell activation