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[DOI] [PMC free article] [PubMed] [Google Scholar] 67.Qin X., Deitch E

Streamlined purification process leads to higher purity and recovery of recombinant proteins from cultured cells

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A MiniTrap desalting column was used to change the buffer of the protein samples to 20 mM Na 2 HPO 4 , p H 7.5, 50 mM NaF, 5% ( w / v ) glycerol containing either 1 mM DTT or 0.5 mM GSH as reducing agents