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The procedure is quick, minimally uncomfortable, and performed under sterile conditions

Once the samples reached confluency the cells were subcultured and all tendon fibroblasts between passages 2 and 4 that had a proper growth rate and normal fibroblast shape were used in the experimentation process

in contrast, the 130 amino acids residues of GhGPX9, 10 may be a mitochondrial or chloroplastic targeting peptide, as predicted by iPSORT and TargetP, which were used to predict the subcellular localisation of eukaryotic proteins ( Differential expression pattern and subcellular localisation of GhGPX products To determine which tissues normally express GhGPX genes, gene-specific primers were used for transcription analysis in roots, stems, flowers, cotyledons and leaves of G