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Description

L-arginine augments endothelium-dependent vasodilation in cholesterol-fed rabbits

After 60 min incubation the samples were washed 2x with 1 ml of binding buffer (125 mM Tris-HCl (pH = 8.0), 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.1% Tween 20), mixed with 1 ug of SNIP1 peptide in 300 ul of binding buffer and rotated for 60 min at 4 C

c , Representative western blot for fibrotic markers in 10 ng/mL PDGF, 10 M Angiotensin II (Ang II), 10 ng/mL CTGF or 1 g/mL lipopolysaccharide (LPS) stimulated LX-2 cells treated with DP (10 M) for 48 h