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Hepatocyte premature senescence is initiated by oxidative stress, along with reduction of IGF-1 during CCl 4 -induced liver fibrogenesis The area density of Masson staining and SA--gal staining showed that with time, CCl 4 initiated hepatic steatosis on Day 6 and the early stage of fibrosis on Day 28

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J Biol Chem 273: 76287636 Hausmann A, Samans B, Lill R, Muhlenhoff U (2008) Cellular and mitochondrial remodeling upon defects in ironsulfur protein biogenesis

At the end point, cells were washed 3 times with saline and stained for 20 min at room temperature using a saline solution containing 0.2 mM l-glutamic acid -(4-methoxy--naphthylamide) (GMNA