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can temporarily reduce immune competence or even disrupt the regulation of systemic inflammation (61)

Neuhaus et al., 2021)

As a proof-of-concept experiment, primary cortical cultures were chronically treated with Nusinersen (NUS) (200 nM), an antisense oligonucleotide that modulates SMN2 splicing and is one of the currently FDA-approved drugs used as therapy for SMA [20, 57], from DIV7 to DIV15 , a period corresponding to neuronal functional maturation in vitro [54] ( Fig.7) which is characterized by significant synaptic development and maturation in cortical cultures