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Tissue homogenization: Frozen tissue of brain was homogenized in 9 volumes of ice-cold tissue lysis buffer containing 150 mM sodium chloride, 1.0% NP-40, 50 mM Tris, pH 8.0 and 1 mM PMSF (phenylmethane sulfonylfluoride) with protease inhibitors (Sigma, Singapore)

Timeline of benefits from Pinealon Within 1-2 weeks of beginning your Pinealon treatment, you should see an increase in your focus and mental clarity less brain fog and more cognitive vigor as well as improvements in mood and stress resilience

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Before initiate the experimental sets, the antioxidant activity of Ab aqueous extract was confirmed by the in vitro assay for total antioxidant activity (TAC) (data not shown)