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To construct an oxidative stress IEC-6 cell model using HO, cells were seeded in 96-well plates and exposed to HO at concentrations of 100, 200, 300, 400, 500, 600, and 700 mol/L

In the mid-to-late 1960s, furosemide was synthetized and quickly gained popularity due to its strong diuretic effects, fast action and effectiveness, especially in treating heart failure, hypertension, edema and kidney failure (Ingram, 1964