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Distinct ATF1 isoforms differ in DNA-binding affinity and subnuclear localization (243), whereas multiple ATF2 variants, including the human isoforms ATF2-sm and ATF2 (SV5) as well as the murine variant Atf2(8,9) (244-246), exhibit reduced transcriptional activity and may alter gene expression by modifying nuclear-cytoplasmic trafficking or by interfering with canonical ATF2/AP-1 dimerization

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TG2-mediated modification of the GST tag caused aggregate formation but did not affect its glutathione binding affinity

The HG + si-NC group (si-NC group) was cultured in medium containing 25 mmol/l glucose for 48 h, with the addition of 5 l of si-NC at a concentration of 20 M for 6 h, followed by culture in medium containing 5.5 mmol/l glucose for 48 h