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However, these in vitro studies often use relatively high Mn concentrations (up to 1 mM), exceeding physiological brain Mn levels, but acute culture systems generally require higher doses to reveal mechanistic effects in the absence of the physiological environment

Step 4: Draw the Correct Volume of Bacteriostatic Water Using a fresh, sterile syringe and needle, carefully draw up the calculated volume of Bacteriostatic Reconstitution Water (bac)

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