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The GHK tag promoted crystallization, with various residues (His, Asp, His/Pro) from symmetry molecules completing the copper(II) square-pyramidal coordination sphere

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Freshly isolated naive CD4 + T cells (0.5 10 6 cells per ml) were activated with plate-bound antibodies and with mouse IL-2 (3 ng/ml -1 ) and human TGF-1 (10 ng/ml -1 ) for iT reg differentiation, with mouse IL-2 (10 ng/ml -1 ) and mouse IL-12 (20 ng/ml -1 ) for T H 1 differentiation, with mouse IL-2 (2 ng/ml -1 ), mouse IL-4 (50 ng/ml -1 ) and anti-mouse IFN- (10 g/ml -1 ) for T H 2 differentiation, or with mouse IL-6 (50 ng/ml -1 ), human TGF-1 (20 ng/ml -1 ), anti-mouse IL-2 (8 g/ml -1 ), anti-mouse IL-4 (8 g/ml -1 ), and anti-mouse IFN- (8 g/ml -1 ) for T H 17 differentiation

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