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Rajabi et al., 2018

However, its impact on endosomal escape requires careful optimization, as PEG can hinder LNP fusion with endosomal membranes, a phenomenon often referred to as the PEG dilemma 174,175,176

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In this approach, a recombinant protein of interest is commonly fused to a variety of tags (including the His6-tag, maltose-binding protein, and GST) to detect, purify, and pull down the interacting partners of a protein of interest