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Collectively, our findings demonstrate that Rylaze-mediated glutamine depletion synergizes with the serine biosynthesis inhibitor BI4916, resulting in the inhibition of AML cell growth and glutathione production, as well as a reduction in protein synthesis, particularly through the inhibition of cap-dependent mRNA translation ( Figure 7 )

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Higher rates of PPP and purine biosynthesis in SCCs were supported by the fact that in vivo 13 C incorporation into the ribose subunit of adenine nucleotides (AXP) was observed in 3 out of 9 SCC, but only 1 out of 9 AdC, and was not observed in the NC counterparts (Fig