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Materials and Methods Cell Culture 3T3 fibroblasts were cultured in Dulbecco's modified Eagle's medium supplemented with 10% foetal calf serum (Invitrogen, San Diego, CA, USA) and antibiotics (25 U/ml penicillin, 25 g/ml streptomycin, and 0.3 g/ml amphotericin B) (Invitrogen, San Diego, CA, USA) in 5% CO 2 in air at 37C in 25 or 75 cm 2 flasks

Download the guide to learn: The top 21 CFR Part 211 sections driving warning lettersand what they reveal about systemic weaknesses Why supplier qualification is likely your biggest unaddressed vulnerability How to tell if your quality unit has functional authority, not just documented authority What regional data integrity disparities tell us about compliance culture Why waiting for regulatory action to seek an external perspective is already too late Six self-assessment questions to pressure-test your own systems today The companies that avoid warning letters aren't luckythey're proactive

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