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Metabolic modulators such as antioxidants, metformin, and melatonin also show potential by modulating mitochondrial and immune pathways

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After centrifugation at 12,000 rpm for 10 min, 300 L of the supernatant was added to each corresponding well of the enzyme label plate, and the OD of each well was measured at 593 nm with a BioTek Epoch

Finally, the relative contributions of direct neuronal regulation versus indirect effects via immune cells (e.g., macrophages) to the observed anti-inflammatory effects remain undetermined