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Both probiotic bacteria significantly upregulated the expression of tight junction proteins compared to untreated mice and, thus, improved intestinal barrier function in the treated mice

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The other predictors of ECOPD reported are poor exercise tolerance as measured by the 6-min walk distance (6MWD) test or the five-times sit-to-stand test (5STS) 31 , reduced hand-grip strength 32 , frailty measured by the 4-meter gait speed test 33 and increased IgE levels 34,35

The tissue was then incubated in Hanks buffered salt solution (HBSS) containing 15 mM HEPES, 50 U/mL collagenase type II (Thermo Fisher 17101015), and 100 U/mL hyaluronidase (Sigma Aldrich H3506) at 37 C for 30 min