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Front Endocrinol (Lausanne) 13:886451

Switching of modA expression from OFF to ON state within the middle ear produces a phenotypic switch in several genes during infection that increases disease severity and permits bacterial persistence by increasing cell adhesion, invasion and robust biofilm formation ( ModA16 ON state, NTHi upregulates multiple iron-acquisition factors including an iron ABC transporter substrate-binding protein, and haem/haemopexin-binding proteins HxuC and HxuB (Tram et al., 2024)

For the proteomic analysis, 300 L aliquots of urine were centrifuged twice at 10,000 g for 20 min at 4C to separate urinary proteins from small molecules using an Amicon Ultra 0.5 ml Centrifugal Filter (10 kDa, UFC501096, Merck Millipore, Darmstadt, Germany) according to the manufacturers instructions

2023;100:e921e931