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In addition, these workers found that antidepressant treatment may lead to defective spindle assembly and altered organelle distribution, with changes in the protein concentrations of small C-terminal domain phosphatases-1 and -3, nuclear mitotic apparatus (NuMa), and calnexin occurring during cell division in vitro

Additionally, in BV2 microglia exposed to isoflurane, MTZ decreased the expression of the protein Iba1 and decreased the production of the proinflammatory mediators interleukin (IL)-1 and IL-18, which isoflurane produces by blocking the activation of the nod-like receptor family protein 3 (NLRP3) inflammasome in BV2 microglia

The samples were homogenized to allow for centrifugation of the supernatant, followed by measurement of AngIV, TNF-alpha, IL-10, and IL-1-beta levels through the use of a sensitive and specific ELISA assay

2024;12:e52897