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Description

Reaction components were 25 M Cp1B or mutants, 5 mM ()- t -ES, 2.5 mM l-Ile, 10 mM ATP and 10 mM MgCl 2

pH was adjusted to 7.2 with CsOH

Fat accumulation in the visceral adipose tissue and liver has been shown to increase the secretion of proinflammatory cytokines, such as tumor necrosis factor (TNF)-, interleukin (IL)-1, IL-6, and leptin, leading to metabolic tissue inflammation and insulin resistance in obese individuals 3,4,5