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The supernatant was added to a reaction mixture consisting of 0.5 ml of 100 mM potassium phosphate buffer (pH 7.0) and 1 ml of 1 M potassium iodide

The most abundant omega-3 fatty acid is docosahexaenoic acid (DHA, 22:6n-3), followed by eicosapentaenoic acid (EPA, 20:5n-3), and docosapentaenoic acid (DPA, 22:5n-3), whereas the main omega-6 fatty acid is arachidonic acid (AA, 20:4n-6)