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Supernatant containing proteins were collected for immunoblotting, extracted proteins (2040 g) were separated by SDS-PAGE (615%) gel and then electroblotted onto Polyvinylidene Fluoride (PVDF) membranes (Amersham Hybond-P, GE-Healthcare Life Science, Pittsburgh, PA, USA)

10.1158/0008-5472.CAN-14-2676 76 LiS.ChenL.JinW.MaX.MaY.DongF.et al (2017)

Nature (2009) 459:52833.10.1038/Nature07999 15 ChangoAPotier De CourcyGBoissonFGuillandJCBarbeFPerrinMOet al5,10-Methylenetetrahydrofolate reductase common mutations, folate status and plasma homocysteine in healthy French adults of the Supplementation en Vitamines et Mineraux Antioxydants (SU.VI.MAX) cohort