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Heatmap generation Heatmap indicating transcript abundance and expression pattern was generated using the HEATMAP hierarchical clustering web tool ( The log2 fold-change values of relative expression levels (the 2 (Ct) value) were used to construct the heatmap

However, it was recently found that preventing accumulation of ferrostatins in lysosomes by reducing the extent of molecular trapping via the lysosomotropic effect improved the potency of ferroptosis inhibitors, which indicates that lysosomes are not critical for ferroptotic suppression [35]

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In summary, we investigated the role of GPX1 in protecting SGNs against oxidative stress by upregulating or inhibiting the expression of GPX1 in SGNs via the GPX1 mimic ebselen or the gpx1 knockout mouse model, and further determined the mechanistic details behind the neuroprotective effect of GPX1 in SGNs against peroxynitrite injury