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The first versions were developed and put in use long before there were FDA regulations on what kinds of clinical trials to do, and what kind of dose-finding data are needed. The new work used a clinical non-inferiority trial to test the immune response of people vaccinated with a standard yellow fever vaccine dose to those who received a one-fifth fractional dose

Recordings of brain swelling were performed in rats before sacrifice after complete calvariectomy was performed (Gojkovic et al., 2021a

General research practice: use a larger gauge like 20G to 22G to draw diluent and add it, then switch to a smaller sterile needle for administration per protocol