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Immunoblotting analysis indicated that 120 mM NaCl elicited a significant decrease in protein levels of ferritin (Figures 1C, D)

Regenerative Biology The growing field of regenerative science has increased attention toward longevity-focused peptide research

The pellet containing Golgi membrane was resuspended using 1 ml of washing buffer (25 mM HEPES-KOH pH 7.4, 3M KCl, 2.5 mM Mg(OAc) 2 , 1 mg/ml soybean trypsin inhibitor), followed by incubation on ice/water for 5 minutes, and then incubated with myristoylated ARF1 (6 g/ml), ARFGAP1 (6 g/ml), BARS (3 g/ml), coatomer (6 g/ml), and GTP (2 mM) at 37C for 30 minutes in 300 l of reaction buffer (25 mM HEPES-KOH pH 7.4, 50 mM KCl, 2.5 mM Mg(OAc) 2 , 1 mg/ml soybean trypsin inhibitor, 1 mg/ml BSA, and 200 mM sucrose)

PMID 14207116