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A handful of other genes, including ODZ4 , coding for cell surface proteins involved in signaling and neuronal path finding, NCAN , a brain-expressed extracellular matrix glycoprotein (rodents with altered NCAN gene function show manic-like behaviors) and ANK3 , a gene involved in localization of sodium channels, have had replicated GWAS support (20, 33, 34, 37, 38)

During brain development there are dramatic changes in the number of synapses in the brain, with different regions of the brain developing at different rates and times (Bourgeois et al., 1994

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The cells were incubated at 37 C, 5% CO 2 , 98% humidified incubator, and were kept sub-confluent by sub-culturing using trypsin