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Description

Based on the copper content measurements, all recombinantly produced proteins ( jr PPO1-wt and five mutants) contain a similar amount of copper (~0.81.1 copper ions per protein), indicating that the changes in activity are not based on the number of copper ions in the active center

Animals All mice were maintained under specific pathogen-free conditions at the Prklinisches Experimentelles Tierzentrum (PETZ), Erlangen, Germany and approved by the local ethics authorities of the Regierung of Unterfranken (#55.2-2532-2-424 and #55.2-2532-2-630)