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Imaging and analysis For immunohistochemical outcome measures, the rostral and caudal medial corpus callosum were visualised using a Nikon A1 confocal microscope (Nikon Corporation, Australia, Coherent Scientific), and a series of 13 images were taken at 0.5 m increments along the z-axis at a magnification of 20 and a numerical aperture of 0.75

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Furthermore, ESM1 expression was found to be upregulated in mouse models of hypoxia-induced retinal vascular neovascularization and laser-induced choroidal vascular neovascularization (47)