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Suspensions were centrifuged at 5,000 g for 5 min at 4C, supernatants were transferred to a new tube, and TCA buffer was added to a final concentration of 33% and incubated at 4C overnight to precipitate histone proteins

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Therefore, preclinical models can help better understand CIPN mechanisms, not only resulting in biomarker discovery that can be translated to the bedside, but also informing therapeutic strategies to prevent and mitigate CIPN that can be tested in humans