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These studies focus on understanding endocrine system interactions and metabolic feedback mechanisms under controlled laboratory conditions and are intended solely for scientific exploration rather than clinical or applied use

Primary antibodies were diluted 1:1000 in Primary Antibody Dilution Buffer (Beyotime, China), as listed in Supplementary Table S13

The preparation of mouse lung cell suspensions was conducted via collagenase digestion of the lungs, with the upper airways removed as previously described 81