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2.8 Western blotting Proteins were extracted from the tissue samples, C2C12 myocytes, or HUVECs using RIPA lysis buffer containing protease and phosphatase inhibitor cocktails (Nacalai Tesque, Kyoto, Japan), separated using SDS-PAGE, and transferred to PVDF membranes

Increased receptor for advanced glycation end products in spermatozoa of diabetic men and its association with sperm nuclear DNA fragmentation

It promotes formation of new blood vessels, reduces oxidative stress markers, and modulates the nitric oxide system (Hsieh et al., 2017)

We hypothesized that the lack of accumulated cysteine and glycine could be due to these metabolites entering alternative metabolic pathways