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Always follow appropriate sterile handling procedures and applicable regulations.

Demir S

Inoculum: [NOTE-A frozen suspension of Lactobacillus leichmannii may be used as the stock culture, provided it yields an inoculum comparable to a fresh culture.] Make a transfer of cells from the Stock culture of Lactobacillus leichmannii to 2 sterile tubes containing 10 mL of the Culture medium each