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GR activity (U/L) = (dOD s / t) x (440 / (OD-CAL - OD-buffer)) x n, where OD-CAL and OD-buffer are the OD 412nm values of the Calibrator and Assay Buffer, t is the reaction time (20 min recommended) and n is the dilution factor

Add 3.0 mL bacteriostatic water to one 10 IU (3.33 mg) vial ~1.11 mg/mL (~1,111 mcg/mL), a practical dilution for accurate dosing

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Biol Trace Elem Res 24:109117 Mischley LK, Conley KE, Shankland EG, Kavanagh TJ, Rosenfeld ME, Duda JE, White CC, Wilbur TK, De La Torre PU, Padowski JM (2016) Central nervous system uptake of intranasal glutathione in Parkinson's disease