Unfused albumin variants or GLP-1 albumin fusions (2 mg/mL) were loaded onto the column in a pH gradient from pH 5.5 to pH 8.8 by injecting a gradual mixture of 20 mM MES/HCl, 140 mM NaCl (Sigma-Aldrich) (pH 5.5) and 20 mM Tris/HCl, 140 mM NaCl (Sigma-Aldrich) (pH 8.8) at a flow rate of 0.5 mL/min for 110 min at room temperature
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Sumanirole is a full-efficacy agonist that, over other dopamine receptors, is highly selective for the D 2 receptor
However, 2-DG also determined a concomitant dose-dependent increase of a ~12 kDa protein that probably represents an aberrant under-glycosylated proIGF-1Ea form
