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For this reason, our 2001 B12 recommendations in What Every Vegan Should Know about Vitamin B12 were focused as much on keeping homocysteine levels low as on preventing the traditional B12 deficiency symptoms of blood and nerve abnormalities

The pre-stained biofilm was then allowed to dry for 15 min at 37C, after which it was dissolved using 80% v/v ethanol and transferred into new 96-well plate for biomass quantification at OD 550 nm using a Tecan plate reader (Infinite M1000 pro)

doi:10.1371/journal.pone.0092573 4.Doyon S

Actinobacteria showed a significantly greater relative abundance in WD control mice compared to all other groups (Fig