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The antitumor activity of the six compounds was evaluated in vitro using the MTT assay against cancer cells (A2780 and A2780cisR) and healthy cells (HEK-293) after 72 h of treatment

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After rinsing with PBS, the sections were incubated with Cy3-conjugated goat-anti-mouse (115-165-003, 1:200, Jackson Immuno Research) for 1 hour at room temperature in the dark

Bidirectional regulatory contradiction: METTL3 simultaneously mediates the m 6 A modification of the 5UTR of dCK mRNA, promotes nuclear export and translation through YTHDC1, and enhances gemcitabine activation, while FTO demethylates dCK, reducing its stability, forming a balanced antagonism that promotes drug resistance/sensitization