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Subsequently, 0.4 mL of a PI staining/lysis solution (0.5% NP-40, 0.5 mM ethylenediaminetetraacetic acid [EDTA]) and 50 L of RNase A (10 mg/mL in TrisEDTA buffer, pH 8.0) solution were added

however, slight angulation of the needle in the caudal direction is necessary to facilitate threading of the catheter

This approach makes intuitive sense because the amount of incretin stimulation needed to maintain a lower weight is probably less than the amount needed to achieve that lower weight in the first place

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