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MPP + treatment significantly reduces TIGAR expression in HT22 cells, downregulates NADPH, GPX4, and GSH levels, upregulates GSSG levels and the GSSG/GSH ratio, decreases adenosine triphosphate (ATP) production, and promotes neuronal ferroptosis, iron-dependent lipid peroxidation, and mitochondrial dysfunction, thereby exacerbating neuronal cytotoxicity

Ong KL, Stafford LK, McLaughlin SA, Boyko EJ, Vollset SE, Smith AE, Dalton BE, Duprey J, Cruz JA, Hagins H, et al

These concentrations have also been used by others 14,34,35 , including ourselves 42 allowing us to compare our results with published studies

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