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doi: 10.1001/jamaneurol.2017.0666 60 CaiBCaiJPLuoYLChenCZhangS

The gel was crushed and the DNA was eluted twice from the crushed gel with 200 L of diffusion buffer (10 mM Tris, 25 mM sodium chloride, 1 mM EDTA, pH 8), at 70 C for 1 hour followed by 30 minutes, with mild shaking (1500 rpm)