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Ribeiro, C.M., Paradiso, A.M., Carew, M.A., Shears, S.B

Advertisement This protocol is an extension of the widely accepted PEN-FAST (penicillin allergy reported by patient, five years or less since reaction, anaphylaxis or angioedema, severe cutaneous adverse reaction, and treatment required for reaction) clinical decision tool that has been used to identify patients with reported penicillin allergy who are appropriate for DOC rather than immediate hypersensitivity skin testing, which recent data suggest has poor positive predictive value in low-risk patients

In order to target roGFP2 into the periplasm, roGFP2 was PCR amplified using the primer pair listed in supplementary table S3 from the pCC_ roGFP2 plasmid as template